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methyl-β-cyclodextrin me-β-cd  (Wacker Chemie)

 
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    Wacker Chemie methyl-β-cyclodextrin me-β-cd
    Methyl β Cyclodextrin Me β Cd, supplied by Wacker Chemie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methyl-%CE%B2-cyclodextrin+me-%CE%B2-cd/methyl+%CE%B2+cyclodextrin/pm40107472-49-0-27
    Average 90 stars, based on 1 article reviews
    methyl-β-cyclodextrin me-β-cd - by Bioz Stars, 2026-11
    90/100 stars

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    Article Title: Enhanced antibacterial efficacy of new benzothiazole phthalimide hybrid compounds/methyl-β-cyclodextrin inclusion complexes compared to the free forms: Insights into the possible mode of action.
    Article Snippet: With the aim to gain further insights into the possible mechanism(s) involved in the cyclodextrin (CD)-mediated enhancement of antimicrobial activity, herein we report the preparation and fully characterization of a series of phenoxy-benzothiazole-phthalimide hybrid compounds and the corresponding complexes with methyl-β-CD (Meβ-CD).. Next, these complexes were tested against selected Gram positive and Gram-negative bacterial strains and the observed antibacterial activity compared to those of the free forms.. Results from 1H NMR and molecular modeling studies showed that in solution the chloro substituted compounds may give inclusion complex of 1:2 Drug-CD stoichiometry besides the 1:1 complexes, while the para-methyl derivative and the unsubstituted compound may provide complexes of 1:1 stoichiometry only.



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    Endocytosis was involved in immune sensitization of monocytes induced by A53T RBC-EVs. A RBC-EVs uptake by THP-1 cells was determined by flow cytometry. Red histogram represented THP-1 cells without RBC-EVs, while blue histogram represented THP-1 cells containing RBC-EVs. The RBC-EVs were labelled with DiO. B – E Effects of endocytosis inhibitors on RBC-EVs uptake in THP-1 cells was determined by flow cytometry . Red histogram represented THP-1 cells co-treated with RBC-EVs and vehicle control, while blue histogram represented THP-1 cells co-treated with RBC-EVs and endocytosis inhibitor. F Quantitative analysis of inhibition ratio of <t>Me-β-CD,</t> Dynasore, Amiloride and Nocodazole. G Levels of oligomeric α-syn in THP-1 cells pretreated with A53T RBC-EVs, along with Me-β-CD, Nocodazole or Amiloride. H Representative confocal image of THP-1 cells incubated with DiI (red)-labelled RBC-EVs after treatment with Dynasore, Nocodazole Me-β-CD and Amiloride, cell nucleus was labelled by Hoechst 33258. Scale bar, 10 µm. I Quantitative analysis of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, IL-8, IFN-γ, IL-2, and IL-12p70, and J anti-inflammatory cytokines IL-4, IL-10, and IL-13 using MSD, released by THP-1 cells pretreated with A53T RBC-EVs alone or with Nocodazole or Me-β-CD, followed by LPS stimulation. N = 3 independent experiments in each group. Values are means ± S.E.M, one-way ANOVA test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001
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    Endocytosis was involved in immune sensitization of monocytes induced by A53T RBC-EVs. A RBC-EVs uptake by THP-1 cells was determined by flow cytometry. Red histogram represented THP-1 cells without RBC-EVs, while blue histogram represented THP-1 cells containing RBC-EVs. The RBC-EVs were labelled with DiO. B – E Effects of endocytosis inhibitors on RBC-EVs uptake in THP-1 cells was determined by flow cytometry . Red histogram represented THP-1 cells co-treated with RBC-EVs and vehicle control, while blue histogram represented THP-1 cells co-treated with RBC-EVs and endocytosis inhibitor. F Quantitative analysis of inhibition ratio of Me-β-CD, Dynasore, Amiloride and Nocodazole. G Levels of oligomeric α-syn in THP-1 cells pretreated with A53T RBC-EVs, along with Me-β-CD, Nocodazole or Amiloride. H Representative confocal image of THP-1 cells incubated with DiI (red)-labelled RBC-EVs after treatment with Dynasore, Nocodazole Me-β-CD and Amiloride, cell nucleus was labelled by Hoechst 33258. Scale bar, 10 µm. I Quantitative analysis of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, IL-8, IFN-γ, IL-2, and IL-12p70, and J anti-inflammatory cytokines IL-4, IL-10, and IL-13 using MSD, released by THP-1 cells pretreated with A53T RBC-EVs alone or with Nocodazole or Me-β-CD, followed by LPS stimulation. N = 3 independent experiments in each group. Values are means ± S.E.M, one-way ANOVA test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

    Journal: Journal of Neuroinflammation

    Article Title: α-Synuclein-containing erythrocytic extracellular vesicles: essential contributors to hyperactivation of monocytes in Parkinson’s disease

    doi: 10.1186/s12974-022-02413-1

    Figure Lengend Snippet: Endocytosis was involved in immune sensitization of monocytes induced by A53T RBC-EVs. A RBC-EVs uptake by THP-1 cells was determined by flow cytometry. Red histogram represented THP-1 cells without RBC-EVs, while blue histogram represented THP-1 cells containing RBC-EVs. The RBC-EVs were labelled with DiO. B – E Effects of endocytosis inhibitors on RBC-EVs uptake in THP-1 cells was determined by flow cytometry . Red histogram represented THP-1 cells co-treated with RBC-EVs and vehicle control, while blue histogram represented THP-1 cells co-treated with RBC-EVs and endocytosis inhibitor. F Quantitative analysis of inhibition ratio of Me-β-CD, Dynasore, Amiloride and Nocodazole. G Levels of oligomeric α-syn in THP-1 cells pretreated with A53T RBC-EVs, along with Me-β-CD, Nocodazole or Amiloride. H Representative confocal image of THP-1 cells incubated with DiI (red)-labelled RBC-EVs after treatment with Dynasore, Nocodazole Me-β-CD and Amiloride, cell nucleus was labelled by Hoechst 33258. Scale bar, 10 µm. I Quantitative analysis of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, IL-8, IFN-γ, IL-2, and IL-12p70, and J anti-inflammatory cytokines IL-4, IL-10, and IL-13 using MSD, released by THP-1 cells pretreated with A53T RBC-EVs alone or with Nocodazole or Me-β-CD, followed by LPS stimulation. N = 3 independent experiments in each group. Values are means ± S.E.M, one-way ANOVA test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

    Article Snippet: Endocytosis inhibitors included Me-β-CD (200 μM, MCE, HY-101461), Amiloride (1 mM, MCE, HY-B0285A), Dynasore (50 μM, Selleck, S8047), and Nocodazole (40 ng/mL, MCE, HY-13520).

    Techniques: Flow Cytometry, Control, Inhibition, Incubation